全文获取类型
收费全文 | 18270篇 |
免费 | 1895篇 |
国内免费 | 2126篇 |
出版年
2024年 | 16篇 |
2023年 | 427篇 |
2022年 | 405篇 |
2021年 | 696篇 |
2020年 | 723篇 |
2019年 | 838篇 |
2018年 | 724篇 |
2017年 | 642篇 |
2016年 | 718篇 |
2015年 | 706篇 |
2014年 | 791篇 |
2013年 | 1186篇 |
2012年 | 737篇 |
2011年 | 758篇 |
2010年 | 622篇 |
2009年 | 882篇 |
2008年 | 933篇 |
2007年 | 961篇 |
2006年 | 949篇 |
2005年 | 860篇 |
2004年 | 830篇 |
2003年 | 745篇 |
2002年 | 711篇 |
2001年 | 627篇 |
2000年 | 575篇 |
1999年 | 489篇 |
1998年 | 396篇 |
1997年 | 322篇 |
1996年 | 357篇 |
1995年 | 300篇 |
1994年 | 300篇 |
1993年 | 305篇 |
1992年 | 269篇 |
1991年 | 201篇 |
1990年 | 190篇 |
1989年 | 162篇 |
1988年 | 139篇 |
1987年 | 118篇 |
1986年 | 95篇 |
1985年 | 143篇 |
1984年 | 102篇 |
1983年 | 61篇 |
1982年 | 66篇 |
1981年 | 52篇 |
1980年 | 31篇 |
1979年 | 23篇 |
1978年 | 18篇 |
1977年 | 22篇 |
1976年 | 16篇 |
1974年 | 13篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
41.
42.
Thea Kristiansen Jens‐Christian Svenning Wolf L. Eiserhardt Dennis Pedersen Hans Brix Søren Munch Kristiansen Maria Knadel César Grández Henrik Balslev 《Journal of Biogeography》2012,39(7):1318-1332
Aim It is a central issue in ecology and biogeography to understand what governs community assembly and the maintenance of biodiversity in tropical rain forest ecosystems. A key question is the relative importance of environmental species sorting (niche assembly) and dispersal limitation (dispersal assembly), which we investigate using a large dataset from diverse palm communities. Location Lowland rain forest, western Amazon River Basin, Peru. Methods We inventoried palm communities, registering all palm individuals and recording environmental conditions in 149 transects of 5 m × 500 m. We used ordination, Mantel tests and indicator species analysis (ISA) to assess compositional patterns, species responses to geographical location and environmental factors. Mantel tests were used to assess the relative importance of geographical distance (as a proxy for dispersal limitation) and environmental differences as possible drivers of dissimilarity in palm species composition. We repeated the Mantel tests for subsets of species that differ in traits of likely importance for habitat specialization and dispersal (height and range size). Results We found a strong relationship between compositional dissimilarity and environmental distance and a weaker but also significant relationship between compositional dissimilarity and geographical distance. Consistent with expectations, relationships with environmental and geographical distance were stronger for understorey species than for canopy species. Geographical distance had a higher correlation with compositional dissimilarity for small‐ranged species compared with large‐ranged species, whereas the opposite was true for environmental distance. The main environmental correlates were inundation and soil nutrient levels. Main conclusions The assembly of palm communities in the western Amazon appears to be driven primarily by species sorting according to hydrology and soil, but with dispersal limitation also playing an important role. The importance of environmental characteristics and geographical distance varies depending on plant height and geographical range size in agreement with functional predictions, increasing our confidence in the inferred assembly mechanisms. 相似文献
43.
In gynodioecious species, females contribute genes to future generations only through ovules, and to persist in populations
they must have a compensatory advantage compared with hermaphrodites that reproduce via ovules and pollen. This compensation
can result from greater fecundity and/or superior success of progeny from females. We examined differences in seed production
and progeny success between females and hermaphrodites in the geophyte Wurmbea biglandulosa to explain the maintenance of females. Females produced more ovuliferous flowers and had more ovules per flower than did
hermaphrodites but this did not necessarily result in greater fecundity, in part because seed production of females was pollen-limited.
Over four years in one population, open-pollinated females produced 1.32 more seeds than open-pollinated hermaphrodites (range
1.09–1.63). In two other populations examined for one year only females produced 1.07 and 0.79 as many seeds as hermaphrodites.
Seed production of open-pollinated females and hermaphrodites was only 55% and 73% that of cross-pollinated plants, respectively,
indicating that both genders were pollen-limited but females more so than hermaphrodites. Open-pollinated seeds from females
were 1.18–1.27 times more likely to germinate than seeds from hermaphrodites. No gender differences existed in seedling growth
or survival. Hermaphrodites were self-compatible, but selfed seed set was only 80% that of crossed seed set. Crossed seed
set of females and hermaphrodites did not differ. Assuming nuclear control of male sterility, relative female fitness is insufficient
to maintain females at their current frequencies of 17%, and substantial female fitness advantages at later life-cycle stages
are required.
Received May 4, 2001 Accepted February 25, 2002 相似文献
44.
Haploid plants were regenerated in vitro from unpollinated ovules of niger (Guizotia abyssinica (L. f.) (Cass.) on Murashige and Skoog nutrient medium (MS) supplemented with 10 μM naphthaleneacetic acid or 10 μM NAA +
1.5 μM kinetin and 30 g/l sucrose. Gamborg (B5) medium was the best for plant regeneration (in comparison with MS, Nitsch
and Nitsch (NN), and Chu (N6) media) from cultured ovules, and 6.66 and 7.33 ovules of JNC-6 and Ootacamund cultivars were
involved in direct plant regeneration on this medium. Matured ovules (ovules collected one day before anthesis or on the day
of anthesis) only responded to cultural regimes and involved in direct plantlet development. Cytological preparation of root
tips and chloroplast counts in the guard cells of leaf stomata of regenerated plants confirmed their haploid nature.
This text was submitted by the authors in English. 相似文献
45.
46.
Cheka Kehelpannala Thusitha Rupasinghe Asher Pasha Eddi Esteban Thomas Hennessy David Bradley Berit Ebert Nicholas J. Provart Ute Roessner 《The Plant journal : for cell and molecular biology》2021,107(1):287-302
Mass spectrometry is the predominant analytical tool used in the field of plant lipidomics. However, there are many challenges associated with the mass spectrometric detection and identification of lipids because of the highly complex nature of plant lipids. Studies into lipid biosynthetic pathways, gene functions in lipid metabolism, lipid changes during plant growth and development, and the holistic examination of the role of plant lipids in environmental stress responses are often hindered. Here, we leveraged a robust pipeline that we previously established to extract and analyze lipid profiles of different tissues and developmental stages from the model plant Arabidopsis thaliana. We analyzed seven tissues at several different developmental stages and identified more than 200 lipids from each tissue analyzed. The data were used to create a web-accessible in silico lipid map that has been integrated into an electronic Fluorescent Pictograph (eFP) browser. This in silico library of Arabidopsis lipids allows the visualization and exploration of the distribution and changes of lipid levels across selected developmental stages. Furthermore, it provides information on the characteristic fragments of lipids and adducts observed in the mass spectrometer and their retention times, which can be used for lipid identification. The Arabidopsis tissue lipid map can be accessed at http://bar.utoronto.ca/efp_arabidopsis_lipid/cgi-bin/efpWeb.cgi . 相似文献
47.
48.
49.
《Journal of molecular biology》2021,433(19):167162
Many proteins that can assemble into higher order structures termed amyloids can also concentrate into cytoplasmic inclusions via liquid–liquid phase separation. Here, we study the assembly of human Golgi-Associated plant Pathogenesis Related protein 1 (GAPR-1), an amyloidogenic protein of the Cysteine-rich secretory proteins, Antigen 5, and Pathogenesis-related 1 proteins (CAP) protein superfamily, into cytosolic inclusions in Saccharomyces cerevisiae. Overexpression of GAPR-1-GFP results in the formation GAPR-1 oligomers and fluorescent inclusions in yeast cytosol. These cytosolic inclusions are dynamic and reversible organelles that gradually increase during time of overexpression and decrease after promoter shut-off. Inclusion formation is, however, a regulated process that is influenced by factors other than protein expression levels. We identified N-myristoylation of GAPR-1 as an important determinant at early stages of inclusion formation. In addition, mutations in the conserved metal-binding site (His54 and His103) enhanced inclusion formation, suggesting that these residues prevent uncontrolled protein sequestration. In agreement with this, we find that addition of Zn2+ metal ions enhances inclusion formation. Furthermore, Zn2+ reduces GAPR-1 protein degradation, which indicates stabilization of GAPR-1 in inclusions. We propose that the properties underlying both the amyloidogenic properties and the reversible sequestration of GAPR-1 into inclusions play a role in the biological function of GAPR-1 and other CAP family members. 相似文献
50.
S. Raj V. Vinod J. Jayakumar P. Suresh A. Kumar R. Biswas 《Letters in applied microbiology》2021,73(1):31-38
Candida species are opportunistic human fungal pathogens that cause acute and chronic infections against which only few antifungal agents are available. Here we have elucidated the antifungal effect of Syzygium samarangense leaf extracts (SSLE). Antifungal activity of SSLE was studied against Candida albicans, C. krusei, C. parapsilosis, C. glabrata, C. auris and C. tropicalis. Following experiments were performed: minimum fungicidal concentration (MFC) determination, agar well disc diffusion assays, fungal morphology analysis using scanning electron microscope (SEM), ex vivo fungal survival assays on porcine tongue and skin and in vivo fungal survival assays using Drosophila melanogaster fly model. Results demonstrated MFC of SSLE ranges between 100 and 125 mg ml−1. SEM images showed cell wall degradation of C. albicans when treated with SSLE. Around 75% decrease in C. albicans viability was observed when infected porcine tongue and skin were treated using SSLE. The C. albicans infected D. melanogaster when fed with SSLE showed significant decrease (around 80%) of fungal count than the infected control. Furthermore, agar plate disc diffusion assays demonstrated that the antifungal activity of SSLE could be due to chalcone, which is one of the active constituents in SSLE. Our study demonstrated that SSLE could be used for the topical treatment of Candida infections. 相似文献